Vitis, apices, micropropagacion, segmentos nodales, explantes axilares, plantulas crecidas in vitro. At both in vitro and ex vitro conditions, root formation was not observed in controls, the media without growth regulators. In tissue culture, the propagated shoots of teak may be rooted ex vitrum like conventional cuttings or in. Qualitative and quantitative changes in putrescine, spermidine, and spermine tissue contents, during the rooting process and their correlation with other biochemical markers and morphological changes, provided the rationale. The ms basal medium containing half strength of macronutrients supplemented with 3% wv sucrose and 0. In vitro rooting could be induced in the rejuvenation and rooting medium, which was 12 om supplemented with 2. Establishment and in vitro clonal propagation of the spanish. The anatomy of normal and hyperhydric in vitro shoots and leaves from micropropagated simmondsia chinensis link. The rooting experiments were conducted in vitro and ex vitro conditions with micropropagated shoots approximately 12 cm long obtained from the fifth subcultures.
In vitro shoot proliferation and in vitro and ex vitro. Dendrobium nobile plantlets derived from seeds germinated in vitro were used with a mean initial height of 1. Efficient method of micropropagation and in vitro rooting. Efficient method of micropropagation and in vitro rooting of. In vitro dendrobium nobile plant growth and rooting in. Krasser article pdf available in gayana botanica 691. Comino crespo, establecimiento in vitro, in vivo, miniesquejes, recalcitrancia. Pdf caracterizacion fisiologica del enraizamiento in. Qualitative and quantitative changes in putrescine, spermidine, and spermine tissue contents, during the rooting process and their correlation with other biochemical markers and morphological changes, provided the rationale for their use during the study of a.
Michael kane environmental horticulture department university of florida rapid clonal in vitro propagation of plants from cells, tissues or organs cultured aseptically on defined media contained in culture vessels maintained under controlled conditions of light and temperature micropropagation in vitro propagation. In vitro clonal propagation and cutting rooting of native cotton gossypium barbadense l. It was hard to survive for seedlings of in vitro rooting after transplanting. Establishment and in vitro clonal propagation of the.
Physiological and developmental problems encountered by in vitro cultured plants michael w. In vitro normal plantlets displayed good development and survived during the acclimatization stage. None of the published protocols fully satis es the requirementsfor commercialapplicationin spite ofthe progress made on tissue culture of teak since the 1970s8,18. This study was carried out to assess in vitro plant growth and rooting of dendrobium nobile seedlings in culture media containing different sucrose concentrations. Ms nutrient media containing iba at all tested concentrations table 1. Consuelo rojasidrogo1, carola cuzquencabrejos1, guillermo e. In vitro hyperhydric plantlets presented numerous anatomical. In vitro shoot proliferation and in vitro and ex vitro root. Anatomy of normal and hyperhydric leaves and shoots of in. Abstract a methodology for the in vitro and in vivo establishment of aniba perutilis, a native colombian tree which is cur. Rooting and acclimatization of apuleia leiocarpa plantlets. The existence of welldeveloped and healthy root system of in vitro plants was a very important step for subsequent acclimatization in conditions ex vitro. Cultivo in vitro del aguacatero persea americana mill.
Initiation of roots emerged 14 days after planting. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Original paper effect of silver nitrate on in vitro root. Skoog, woody plant medium, mascarenhas, enraizamiento, hormonas. Physiological and developmental problems encountered by in. During the rooting testing process, the culture medium used was the one formulated by. An in vitro rooting protocol for producing nothofagus nervosa microplants using successive steps during tissue culture is reported.
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